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KMID : 0352720160400040395
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Journal of Ginseng Research
2016 Volume.40 No. 4 p.395 ~ p.399
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Discrimination of Korean ginseng (Panax ginseng Meyer) cultivar Chunpoong and American ginseng (Panax quinquefolius) using the auxin repressed protein gene
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Kim Jong-Hak
Kim Min-Kyeoung
Hongtao Wang
Lee Hee-Nyeong
Chi-Gyu Jin
Kwon Woo-Saeng
Yang Deok-Chun
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Abstract
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Background: Korean ginseng (Panax ginseng) is one of the most important medicinal plants in the Orient. Among nine cultivars of P. ginseng, Chunpoong commands a much greater market value and has been planted widely in Korea. Chunpoong has superior quality “Chunsam” (1st grade ginseng) when made into red ginseng.
Methods: A rapid and reliable method for discriminating the Chunpoong cultivar was developed by exploiting a single nucleotide polymorphism (SNP) in the auxin repressed protein gene of nine Korean ginseng cultivars using specific primers.
Results: An SNP was detected between Chunpoong and other cultivars, and modified allele-specific primers were designed from this SNP site to specifically identify the Chunpoong cultivar and P. quinquefolius via multiplex polymerase chain reaction (PCR).
Conclusion: These results suggest that great impact to prevent authentication of precise Chunpoong and other cultivars using the auxin repressed protein gene. We therefore present an effective method for the authentication of the Chunpoong cultivar of P. ginseng and P. quinquefolius.
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KEYWORD
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multiplex PCR, Panax ginseng, P. quinquefolius, polymorphism
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